1,25-Dihydroxyvitamin D Alters Lipid Metabolism And Epithelialto- Mesenchymal Transition In Metastatic Epithelial Breast Cancer Cells

Barnard, Alle N. M.S., Purdue University, August 2014. 1,25-dihydroxyvitamin D alters lipid metabolism and epithelial-to-mesenchymal transition in metastatic epithelial breast cancer cells. Major Professor: Dorothy Teegarden. Evidence suggests that high vitamin D status (marked by serum 25hydroxyvitamin D, 25(OH)2D) is associated with a decreased risk of breast cancer. It has been established that 1,25-dihydroxyvitamin D (1,25(OH)2D) can alter glycolysis and the Krebs cycle of breast cancer cells (Jiang et al., 2010; Zheng et al., 2013) but little information is available on 1,25(OH)2D's alterations of lipid metabolism in breast cancer cells. Thus, the current research investigates if there was an effect of 1,25(OH)2D on proteins that regulate lipid metabolism in MCF10A, MCF10A-ras, MCF10CA1h, and MCF10CA1a epithelial breast cancer cells. While no significant effects were seen at 24 hours of treatment with 10 nM of 1,25(OH)2D, several changes were see at 48 hours with the most significant effects in the MCF10CA1a line. With 48 hour treatment of 1,25(OH)2D there was a significant increase of fatty acid synthase (FAS), acylCoA cholesterol acyltransferase 1 (ACAT1), insulin-inducing gene 2 (Insig2), perilipin 2 (PLIN2), and a non-significant trend towards an increase in stearoylCoA desaturase 1 (SCD1) in the MCF10CA1a line. Additionally, there was a non-significant trend towards a decrease in carnitine palmitoyl transferase 1a